Non Susceptibility of Neonatal and Adult Rats against the Middle East Respiratory Syndrome Coronavirus.
Identifieur interne : 001093 ( Main/Exploration ); précédent : 001092; suivant : 001094Non Susceptibility of Neonatal and Adult Rats against the Middle East Respiratory Syndrome Coronavirus.
Auteurs : Naoko Iwata-Yoshikawa ; Shuetsu Fukushi ; Aiko Fukuma ; Tadaki Suzuki ; Makoto Takeda ; Masato Tashiro ; Hideki Hasegawa ; Noriyo NagataSource :
- Japanese journal of infectious diseases [ 1884-2836 ] ; 2016.
Descripteurs français
- KwdFr :
- Administration par voie nasale, Animaux, Coronavirus du syndrome respiratoire du Moyen-Orient (croissance et développement), Femelle, Grossesse, Humains, Immunohistochimie, Lignée cellulaire, Poumon (virologie), Rats de lignée F344, Rats de lignée LEW, Résistance à la maladie, Spécificité d'hôte, Tropisme viral.
- MESH :
- croissance et développement : Coronavirus du syndrome respiratoire du Moyen-Orient.
- virologie : Poumon.
- Administration par voie nasale, Animaux, Femelle, Grossesse, Humains, Immunohistochimie, Lignée cellulaire, Rats de lignée F344, Rats de lignée LEW, Résistance à la maladie, Spécificité d'hôte, Tropisme viral.
English descriptors
- KwdEn :
- MESH :
- growth & development : Middle East Respiratory Syndrome Coronavirus.
- virology : Lung.
- Administration, Intranasal, Animals, Cell Line, Disease Resistance, Female, Host Specificity, Humans, Immunohistochemistry, Pregnancy, Rats, Inbred F344, Rats, Inbred Lew, Viral Tropism.
Abstract
The present study examined the susceptibility of rats to the Middle East respiratory syndrome coronavirus (MERS-CoV) and determined whether this animal is a suitable model for MERS-CoV infection. Immunohistochemical analysis identified dipeptidyl peptidase 4 (DPP4), a known receptor for MERS-CoV on type I pneumocytes from infected rats. Whereas adult rats developed antibodies against MERS-CoV spike protein after intranasal inoculation, there was no evidence of viral replication in the lungs of adult, young, or neonatal rats after intranasal inoculation with MERS-CoV. In addition, human DPP4-expressing rat kidney fibroblasts, but not rat DPP4-expressing cells, were susceptible to MERS-CoV. Taken together, these results suggest that the rat is not a useful animal model for studying MERS-CoV infection.
DOI: 10.7883/yoken.JJID.2015.589
PubMed: 27000459
Affiliations:
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Le document en format XML
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Hasegawa</name></author><author><name sortKey="Nagata, Noriyo" sort="Nagata, Noriyo" uniqKey="Nagata N" first="Noriyo" last="Nagata">Noriyo Nagata</name></author></analytic><series><title level="j">Japanese journal of infectious diseases</title><idno type="eISSN">1884-2836</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Administration, Intranasal</term><term>Animals</term><term>Cell Line</term><term>Disease Resistance</term><term>Female</term><term>Host Specificity</term><term>Humans</term><term>Immunohistochemistry</term><term>Lung (virology)</term><term>Middle East Respiratory Syndrome Coronavirus (growth & development)</term><term>Pregnancy</term><term>Rats, Inbred F344</term><term>Rats, Inbred Lew</term><term>Viral Tropism</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Administration par voie 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Intranasal</term><term>Animals</term><term>Cell Line</term><term>Disease Resistance</term><term>Female</term><term>Host Specificity</term><term>Humans</term><term>Immunohistochemistry</term><term>Pregnancy</term><term>Rats, Inbred F344</term><term>Rats, Inbred Lew</term><term>Viral Tropism</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Administration par voie nasale</term><term>Animaux</term><term>Femelle</term><term>Grossesse</term><term>Humains</term><term>Immunohistochimie</term><term>Lignée cellulaire</term><term>Rats de lignée F344</term><term>Rats de lignée LEW</term><term>Résistance à la maladie</term><term>Spécificité d'hôte</term><term>Tropisme viral</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The present study examined the susceptibility of rats to the Middle East respiratory syndrome coronavirus (MERS-CoV) and determined whether this animal is a suitable model for MERS-CoV infection. Immunohistochemical analysis identified dipeptidyl peptidase 4 (DPP4), a known receptor for MERS-CoV on type I pneumocytes from infected rats. Whereas adult rats developed antibodies against MERS-CoV spike protein after intranasal inoculation, there was no evidence of viral replication in the lungs of adult, young, or neonatal rats after intranasal inoculation with MERS-CoV. In addition, human DPP4-expressing rat kidney fibroblasts, but not rat DPP4-expressing cells, were susceptible to MERS-CoV. 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